Human/Mouse/Rat Phospho-GSK3a (S21) and Total GSK3a PharmaGenie ELISA Kit (SBRS1843)

The dual-analyte format quantifies phosphorylated GSK3α at Ser21 alongside total GSK3α from the same well, enabling direct normalization of phosphorylation status to total protein levels. AKT-mediated phosphorylation at Ser21 inhibits GSK3α activity, so this kit from Assay Genie is widely applied to insulin signaling, PI3K/AKT pathway activation, Wnt/β-catenin regulation, and cellular responses to growth factors or oxidative stress. Researchers studying diabetes, neurodegeneration, and oncology commonly use this ELISA with cell lysates and tissue lysates prepared from human, mouse, and rat models, allowing cross-species comparison without switching assays. The phospho-specific capture antibody selectively recognizes GSK3α only when phosphorylated at Ser21, while the matched total-protein readout corrects for variation in expression. Reconstituted standards should be aliquoted and stored frozen to limit freeze-thaw cycles and preserve phospho-epitope integrity, and the microplate should be equilibrated to room temperature before running the assay.