Human/Mouse/Rat Phospho-Erk2 (T185/Y187) PharmaGenie ELISA Kit (SBRS1820)

The Phospho-Erk2 (T185/Y187) PharmaGenie ELISA from Assay Genie quantifies the dually phosphorylated, catalytically active form of MAPK1, a central node in the RAS-RAF-MEK-ERK signalling cascade. The assay is optimised for cell lysate and tissue lysate workflows, and cross-reactivity has been demonstrated against mouse and rat Erk2 orthologues, supporting translational pathway studies in standard preclinical models. Reconstituted standards and detection antibody aliquots are stored at -20 °C between uses, while the pre-coated microplate is supplied ready for immediate assay setup. Common research applications include profiling growth-factor-induced MAPK activation, characterising oncogenic pathway reactivation, and measuring pharmacodynamic responses to small-molecule MEK or RAF inhibitors.