Human/Mouse/Rat Phospho-Erk1 (T202/Y204)+ Phospho-Erk2 (T185/Y187) PharmaGenie ELISA Kit (SBRS1822)

The MAPK/ERK signaling cascade regulates proliferation, differentiation, and survival. This phosphorylation ELISA from Assay Genie selectively quantifies activated ERK1 at Thr202/Tyr204 and ERK2 at Thr185/Tyr187, the dual phosphorylation motifs generated by MEK1/2. Researchers commonly apply it to study growth factor responses, oncogenic Ras/Raf signaling, and pharmacological MEK inhibition in cancer and inflammation models. Lysate preparation uses RIPA buffer supplemented with protease and phosphatase inhibitor cocktails to preserve the phospho-epitope. The kit includes a pre-coated microplate and reagents sufficient for 96 assays, supporting high-throughput screening of pathway modulation across treated versus untreated conditions.