Human/Mouse/Rat Phospho-ERK1/2 (T202/Y204)Cell-Based ELISA

The Phospho-ERK1/2 (T202/Y204) Cell-Based ELISA from Assay Genie selectively detects ERK1 and ERK2 kinases only when dual-phosphorylated at threonine 202 and tyrosine 204, the canonical activation sites of the MAPK/MEK signaling cascade. This quantitative assay measures endogenous phospho-ERK1/2 levels in cell lysates and tissue lysates, with validated cross-reactivity across human, mouse, and rat samples, supporting translational research in oncology, neurodegeneration, and inflammatory disease. The kit is used to monitor MEK/ERK pathway activation following growth factor stimulation, cytokine treatment, or exposure to small molecule inhibitors such as MEK and BRAF inhibitors. The colorimetric readout in a microplate format reduces variability compared with Western blot analysis and eliminates the need for radioactive substrates. Each well plate supports 96 assays, with reagents stored at -20 degrees C for long-term stability. Avoid repeated freeze-thaw cycles and protect detection antibodies from light. The kit supports time-course studies, dose-response experiments, and in vitro screening of MAPK pathway modulators.