Human/Mouse/Rat Phospho-Beclin-1 (S234) and Total Beclin-1 PharmaGenie ELISA Kit (SBRS1762)

Beclin-1 sits at the heart of autophagy initiation, where it nucleates the PI3K class III complex that drives autophagosome formation. Phosphorylation at Ser234 is a regulatory modification studied to understand how cells control autophagosome biogenesis under nutrient stress, growth factor signaling, and disease states.

The Human/Mouse/Rat Phospho-Beclin-1 (S234) and Total Beclin-1 PharmaGenie ELISA Kit from Assay Genie provides a dual readout, capturing both the phosphorylated fraction at S234 and the total Beclin-1 protein from the same sample, so the phosphorylation ratio can be calculated per specimen rather than inferred across plates.

Built on cell lysates and tissue lysates as the input matrix, the assay is compatible with cultured mammalian cells and dissected tissue prepared through standard lysis workflows. Each kit ships with reagents sufficient for 96 assays in a microplate format, supporting full-plate runs alongside appropriate controls. Beclin-1 is monitored via a colorimetric readout, and the matched phospho and total design supports studies in autophagy signaling, cancer biology, neurodegeneration, and metabolic research where Beclin-1 activity reflects cellular homeostasis.

Researchers often normalize the phospho-S234 signal to the total Beclin-1 signal to follow pathway activation across time-course experiments, dose-response curves, or genetic manipulation studies. The Human, Mouse, and Rat reactivity profile enables translational work using rodent models alongside human cell lines within a single assay platform, and the kit format keeps lysate input volumes modest for downstream replicate sampling.