Human/Mouse/Rat Phospho-AKT (S473) PharmaGenie ELISA Kit (SBRS1743)

The PI3K/AKT/mTOR signaling cascade is read out at the serine 473 phosphorylation site of AKT (also called Protein Kinase B), and this ELISA kit provides a quantitative measure of that activation mark. mTORC2 is the principal kinase that phosphorylates AKT at S473 in response to growth factors, insulin, and cytokines, making this residue a widely used indicator of pathway engagement in oncology, metabolic disease, and kinase inhibitor profiling workflows.

The kit accepts cell lysates and tissue lysates, with validated reactivity across human, mouse, and rat samples so cell-line work can be cross-referenced with rodent model findings. A 96 assays plate format supports medium-throughput screening, and unused reagents should be stored refrigerated in a single-use aliquot workflow to preserve activity across freeze-thaw cycles.

Researchers commonly pair phospho-S473 measurements with total AKT quantification and downstream effector readouts such as phospho-GSK3β or phospho-S6K1 to resolve pathway activation state. Supplied by Assay Genie as part of the PharmaGenie line, the kit fits kinase inhibitor screening, target engagement studies, and translational biomarker work in academic and pharmaceutical pipelines.