Human/Mouse/Rat Phospho-AKT (S473) and Total Akt PharmaGenie ELISA Kit (SBRS1745)

The Phospho-AKT (S473) and Total Akt PharmaGenie ELISA Kit from Assay Genie enables simultaneous quantification of phosphorylated AKT at serine 473 alongside total AKT protein from the same lysate preparation, eliminating the need to run parallel plates for normalization. AKT (Protein Kinase B) is a central node in the PI3K/AKT/mTOR cascade, and S473 phosphorylation by mTORC2 is widely used as a marker of pathway activation in studies of insulin signaling, growth factor response, and cell survival. Researchers apply this dual ELISA to cancer signaling, metabolic disease, neurodegeneration, and drug response profiling, using cell lysates or tissue lysates as input. The microplate-based format delivers reproducible quantitative comparisons of phosphorylated versus total AKT levels across human, mouse, and rat samples, supporting pathway analysis in cell biology and translational research.