Human/Mouse Phospho-Stat 1 (Y701) and Total Stat 1 PharmaGenie ELISA Kit (SBRS1983)

Simultaneous quantification of phosphorylated STAT1 at tyrosine 701 and total STAT1 protein is supported by this dual-analyte ELISA kit, enabling normalization of activation status against total protein abundance in a single experimental workflow. The assay is calibrated for human samples, with the kit designation also indicating mouse cross-reactivity for translational research applications. Cell lysates and tissue lysates serve as validated input material, addressing studies of JAK-STAT pathway activation triggered by interferon, cytokine, or growth factor signaling. The Colorimetric readout produces absorbance-based data across 96 assays per kit, allowing multiple biological replicates and treatment conditions to be processed together. Each plate is configured to discriminate the phospho-Y701 epitope from total STAT1, helping researchers distinguish genuine pathway activation from changes in overall STAT1 expression. Provided by Assay Genie as a complete kit, the reagents are intended for research use only and serve signal transduction, oncology, immunology, and cell biology laboratories investigating STAT1-driven responses.