Human/Mouse Phospho-Stat 1 (S727) PharmaGenie ELISA Kit (SBRS1980)

From Assay Genie, this Phospho-Stat 1 (S727) PharmaGenie ELISA Kit is a cell-based phosphorylation immunoassay that quantitatively measures STAT1 phosphorylated at the serine 727 residue, a regulatory site activated downstream of JAK-STAT signaling in response to interferon, growth factor, and cytokine stimuli. The 96-well microplate format uses a colorimetric readout and is validated for use with cell lysates and tissue lysates from human samples, with cross-reactivity confirmed for mouse specimens, supporting pathway-focused studies in immunology, oncology, and signal transduction research.

The capture antibody is specific for the S727 phospho-epitope, allowing researchers to distinguish serine 727 activation from the more commonly studied Y701 tyrosine phosphorylation event. Store the microplate strips under refrigerated conditions and the detection antibody at -20°C for long-term stability; allow all reagents to equilibrate to room temperature before beginning the assay.