Human/Mouse Phospho-Rictor (T1135) PharmaGenie ELISA Kit (SBRS1948)

This phosphorylation ELISA from Assay Genie is built around a site-specific antibody that recognizes Rictor only when threonine 1135 is phosphorylated, a regulatory residue within the mTORC2 complex that governs Akt Ser473 phosphorylation, cytoskeletal remodeling, and cell survival signaling. Rictor runs at approximately 192 kDa on western blots, providing a useful reference for lysate preparation and normalization. Researchers working on insulin signaling, cancer cell proliferation, or metabolic disease use the kit to quantify pathway activation in cell and tissue lysates, often in parallel with total Rictor and phospho-Akt western blots. The microplate format supports 96 assays per run, the recommended sample load is 100 µL of diluted lysate, and capture is human-specific with cross-reactivity to mouse ortholog, making it useful for parallel human and mouse model studies. Unopened kits should be stored refrigerated and protected from light, with lyophilized reagents remaining stable through multiple freeze-thaw cycles when aliquoted. For research use only; not intended for diagnostic applications.