Human/Mouse Phospho-p38 alpha (T180/Y182) PharmaGenie ELISA Kit (SBRS1915)

This phosphorylation-specific immunoassay from Assay Genie quantifies the activated form of p38 MAPK (mitogen-activated protein kinase 14) by detecting dual phosphorylation at threonine 180 and tyrosine 182. The p38 signaling cascade governs cellular responses to stress, inflammatory cytokines, UV exposure, and osmotic shock, making this kit valuable for pathway-focused research in oncology, immunology, and neurodegeneration.

Intended for use with cell lysates and tissue lysates, the plate format accommodates multiple experimental conditions, dose-response studies, and time-course analyses of kinase activation. Each kit supports 96 individual assays, providing sufficient throughput for screening pharmacological inhibitors, validating upstream signaling events, or profiling MAPK pathway engagement across treated versus untreated samples.

Typical research applications include:

• Measuring p38 pathway activation in response to TNF-alpha, IL-1, or anisomycin stimulation
• Validating small-molecule kinase inhibitors or pathway modulators in vitro
• Profiling stress-activated kinase activity in disease models
• Confirming phosphorylation changes detected by western blot using an orthogonal antibody-based readout

The kit is intended for research use only and supports laboratories studying signal transduction, drug discovery, and cellular stress biology.