Human/Mouse Phospho-MARCKS (S152/156) and Total MARCKS PharmaGenie ELISA Kit (SBRS1888)

The MARCKS protein (Myristoylated Alanine-Rich C-Kinase Substrate) is a major substrate of protein kinase C, and phosphorylation at Ser152/156 functions as a quantitative readout of PKC activity in cells and tissues. This dual-target ELISA from Assay Genie simultaneously measures phospho-MARCKS (S152/156) and total MARCKS within the same sample, allowing researchers to normalize phosphorylation signals against total protein expression - an essential control for accurate cell signaling studies.

Validated for cross-reactivity with both human and mouse samples, the assay is optimized for cell lysates and tissue lysates and runs on a standard microplate workflow. Common research applications include PKC pathway investigation in neuroscience, cancer cell migration, phagocytosis, cytoskeletal remodeling, and drug screening for kinase modulators. The dual-analyte format is particularly valuable for time-course studies comparing basal versus stimulated phosphorylation states.

For handling, unopened kits should be stored at -20°C and remain stable through multiple freeze-thaw cycles when components are aliquoted. Researchers can pair this ELISA with companion reagents offered through the Assay Genie portfolio to build complete signaling studies.