Human/Mouse Phospho-4E-BP1 (T36) and Total 4E-BP1 PharmaGenie ELISA Kit (SBRS1737)

This phosphorylation ELISA from Assay Genie enables simultaneous measurement of 4E-BP1 phosphorylated at threonine 36 alongside total 4E-BP1 protein in the same sample well, supporting pathway analysis of mTORC1 signaling in translational control research.

4E-BP1 acts as a key downstream effector of mTOR, and its multisite phosphorylation status governs cap-dependent translation initiation events frequently probed in studies of cancer cell proliferation, metabolic disease, and growth factor responses. The kit is supplied in a 96 assays format and accepts cell lysates or tissue lysates, making it appropriate for cultured cell models and dissected tissue specimens.

Researchers investigating nutrient sensing, insulin signaling, or oncogenic PI3K/AKT/mTOR pathway activity can use the paired phospho-and-total readout to normalize phosphorylation levels against total protein expression, controlling for sample-to-sample loading variation. The colorimetric readout is compatible with standard microplate readers used in routine laboratory workflows.