Human, Mouse and Rat Phosphotyrosine FRS2 PharmaGenie ELISA Kit (SBRS1834)

The kit specifically recognizes the tyrosine-phosphorylated form of Fibroblast Growth Factor Receptor Substrate 2 (FRS2), a membrane-associated docking protein that becomes phosphorylated at multiple tyrosine residues upon activation of FGF receptors, neurotrophin receptors, and selected cytokine receptors. By selectively capturing phospho-FRS2 from cell lysates and tissue lysates, this microplate-based ELISA enables quantitative measurement of pathway activation and complements western blot workflows that often consume larger amounts of lysate and lack inter-experiment standardization.

Assay Genie validates cross-reactivity with human, mouse, and rat samples, supporting comparative studies of receptor tyrosine kinase signaling in developmental biology, neuronal differentiation, angiogenesis, and oncogenic FGFR research. For reproducible results, prepare lysates fresh or store aliquots at low temperature, limit freeze-thaw cycles, and equilibrate all microplate components to room temperature before initiating the colorimetric assay readout.