Human, Mouse and Rat Phosphotyrosine FAK PharmaGenie ELISA Kit (SBRS1824)

Quantifying tyrosine-phosphorylated Focal Adhesion Kinase (FAK) in experimental samples, this immunoassay kit from Assay Genie measures activated FAK in cell lysates and tissue lysates. Phosphorylation of FAK at key tyrosine residues, particularly the Tyr397 autophosphorylation site, is a well-established marker of integrin-mediated adhesion signaling and is frequently monitored in studies of cell migration, cytoskeletal dynamics, and tumor invasion.

Validated for use with human, mouse, and rat samples, the assay enables cross-species investigation in oncology, fibrosis, and cardiovascular research where altered FAK activity is of interest. The microplate-format package provides reagents for 96 individual determinations, supporting medium-throughput experiments that examine FAK pathway modulation in response to kinase inhibitors, growth factor stimulation, or extracellular matrix engagement.

Cell lysates and tissue lysates should be prepared with standard lysis buffers containing phosphatase inhibitors to preserve phosphorylation integrity during processing. Researchers apply this ELISA to validate phosphoproteomic screening hits, compare baseline versus stimulated FAK activation, and track pharmacodynamic responses in drug discovery programs targeting the FAK signaling axis.