Human, Mouse and Rat Phospho-TBK1 (S172) PharmaGenie ELISA Kit (SBRS1999)

The Phospho-TBK1 (S172) ELISA from Assay Genie quantitatively measures activated TANK-binding kinase 1, the central mediator of type I interferon and NF-kB signaling. This phosphorylation-specific immunoassay selectively detects the activating S172 site that gates downstream IRF3 phosphorylation and IFN-beta transcription. Researchers investigating antiviral innate immunity, TLR3/4 signaling, TRAF-associated pathways, and autophagy initiation use this kit to monitor TBK1 pathway activation in cell culture and tissue models. The assay accommodates cell lysates and tissue lysates from human, mouse, and rat samples, supporting translational immunology and inflammation research. Each kit includes pre-coated microplate wells, capture and detection antibodies, reference standard, and buffers for a 96-assay workflow. Components should be stored refrigerated to preserve phospho-epitope integrity; aliquot working standards and keep on ice during the procedure to limit dephosphorylation. For research use only; not validated for diagnostic or clinical applications.