Human, Mouse and Rat Phospho-TBK1 (S172) and Total TBK1 PharmaGenie ELISA Kit (SBRS2000)

From Assay Genie, the Phospho-TBK1 (S172) and Total TBK1 PharmaGenie ELISA Kit enables simultaneous quantification of phosphorylated TBK1 at serine 172 alongside total TBK1 protein levels, allowing researchers to normalize pathway activation against total kinase expression in the same sample. TBK1 (TANK-binding kinase 1) is a serine/threonine kinase central to innate immune signaling, where S172 autophosphorylation marks its activated state during IRF3 and NF-κB pathway induction downstream of pattern recognition receptors. The dual-analyte format supports studies of antiviral responses, type I interferon signaling, and inflammatory pathway modulation using human cell lysates and tissue lysates. The microplate-based workflow can be paired with orthogonal validation by western blot for confirmatory analysis. Use 50 µL of sample per well when running the assay and store components refrigerated at 4 degrees C; protect antibody conjugates from light to preserve activity. The assay is intended for research use only across immunology, cell signaling, and kinase biology laboratories focused on innate immunity, viral defense, and drug mechanism studies.