Human, Mouse and Rat Phospho-PLCG1 (Tyr771) and Total PLCG1 PharmaGenie ELISA Kit (SBRS1933)

Receptor tyrosine kinase signaling through PLCG1 (Phospholipase C Gamma 1) drives calcium and DAG-mediated second messenger cascades downstream of growth factor receptors. The Assay Genie Phospho-PLCG1 (Tyr771) and Total PLCG1 PharmaGenie ELISA Kit quantifies phosphorylation at the Tyr771 activation site alongside total PLCG1 protein in the same 96 assays run, enabling direct normalization of pathway activation across human, mouse, and rat samples.

Compatible with both cell lysates and tissue lysates, the kit supports translational research programs that bridge in vitro cell line work with in vivo animal tissue analysis. Phosphorylation at Tyr771 is a canonical activation marker for PLCG1 and is widely used as a proxy for upstream receptor tyrosine kinase activity, including signaling through EGFR, PDGFR, and FGFR.

Research applications include investigation of oncogenic signaling in EGFR-driven cancers, characterization of immune receptor responses, drug screening campaigns targeting the phosphoinositide pathway, and studies of cellular responses to growth factor stimulation. The dual phospho/total readout on a single plate reduces inter-well variability and provides an internal reference for comparing treatment conditions across experiments.