Human, Mouse and Rat Phospho-PDK1 (Ser241) PharmaGenie ELISA Kit (SBRS1928)

Built around the autophosphorylation site that keeps 3-phosphoinositide-dependent protein kinase 1 switched on, this Assay Genie kit provides a quantitative readout of PDK1 phosphorylated at Ser241 in cell lysates and tissue lysates. The 96 assays format is set up so researchers can profile PI3K/Akt signalling in parallel across human, mouse, and rat samples, supporting pathway studies in cancer metabolism, insulin signalling, and neurodegeneration model systems.

Detection uses a chromogenic, plate-based immunoassay read on a standard microplate reader, and the kit is intended for in vitro research use only. Researchers prepare lysates under phosphatase-inhibitor conditions to preserve the Ser241 phosphorylation mark before adding sample to the pre-coated plate. Each kit includes the immunoassay components required for the complete plate workflow.

Store the kit refrigerated between uses; allow plates and buffers to equilibrate to room temperature before running the assay. The product is intended for laboratory research use, not for diagnostic procedures.