Human, Mouse and Rat Phospho-AMPKa1 (S487) and Total AMPKa1 PharmaGenie ELISA Kit (SBRS1748)

This dual-analyte ELISA kit from Assay Genie quantifies phosphorylated AMPKα1 at serine 487 alongside total AMPKα1 protein in a single 96-assay microplate run, enabling normalization of phosphorylation status to total kinase expression without parallel control plates. The kit is validated for cell lysates and tissue lysates and reacts with human, mouse, and rat samples, making it applicable in metabolic disease research, mTOR and AMPK signaling studies, insulin sensitivity investigations, and autophagy regulation work. Each plate is processed colorimetrically, with capture antibodies specific for the α1 subunit that distinguish it from the β2 isoform. Researchers studying cancer cell metabolism, exercise physiology, type 2 diabetes mechanisms, or hepatic energy homeostasis can use the assay to compare basal and stimulated S487 phosphorylation across treatment conditions. Sufficient reagents are included for 96 assays, and the kit is supplied ready for standard laboratory workflows involving lysate preparation, incubation, and absorbance readout. Results are reported as ratios or absolute units depending on the standard curve generated during each run, supporting both qualitative pathway surveys and quantitative comparisons between experimental groups.