Human Mothers against decapentaplegic homolog 7 (SMAD7) ELISA Kit

A sandwich ELISA from Assay Genie for the quantitative measurement of human Mothers against decapentaplegic homolog 7 (SMAD7) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The assay has a detection range of 0.156–10 ng/mL and a sensitivity of 0.1 ng/mL. Reported intra-assay CV is 6.3% and inter-assay CV is 9.7%.

Linearity was assessed across 1:2, 1:4, 1:8 and 1:16 dilutions for serum (80–92%, 93–103%, 103–114%, 103–115%), EDTA plasma (88–100%, 104–114%, 81–91%, 99–109%) and heparin plasma (97–110%, 95–106%, 102–111%, 92–102%). Recovery averaged 106% for serum (range 100–112%) and 108% for plasma (range 102–114%). The kit is specific for natural and recombinant human SMAD7 and is intended for research use only. SMAD7 is described as an antagonist of TGF-beta signaling that inhibits TGF-beta and activin signaling by associating with their receptors and preventing SMAD2 access; it also functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex, recruits the PPP1R15A-PP1 complex to TGFBR1 to promote its dephosphorylation, and positively regulates PDPK1 kinase activity by stimulating its dissociation from YWHAQ. Subcellular localisation is reported as nucleus and cytoplasm, and the listed research area is epigenetics.