Human Mothers against decapentaplegic homolog 6 (SMAD6) ELISA Kit

Designed for the quantitative measurement of Mothers against decapentaplegic homolog 6 (SMAD6) in human samples, this Sandwich ELISA from Assay Genie provides a reliable tool for research into TGF-β and BMP signaling pathways. SMAD6 is an inhibitory SMAD that antagonizes signaling by TGF-beta type 1 receptor superfamily members and selectively inhibits BMP signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. The kit is validated for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, with reactivity confirmed for human samples and specificity for natural and recombinant human SMAD6.

The assay delivers a detection range of 31.2-2000pg/mL and a sensitivity of 8.6pg/mL. Detection is performed by ELISA in a sandwich format, and SMAD6 is detected in its nuclear subcellular location. Known synonyms include SMAD family member 6, SMAD 6, MAD homolog 6, and MADH6, and the protein is catalogued under UniProt O43541. SMAD6 has been implicated in conditions such as cancer, fibrosis, and autoimmune disorders through dysregulation of the TGF-β pathway, supporting studies of cell growth, differentiation, and development, as well as investigations into potential therapeutic interventions. For research use only.