Human Mothers against decapentaplegic homolog 4 (SMAD4) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is intended for the quantitative detection of Mothers against decapentaplegic homolog 4 (SMAD4) in human samples. Also known by synonyms including DPC4, MADH4, MAD homolog 4, SMAD family member 4, and deletion target in pancreatic carcinoma 4, SMAD4 is a key signaling protein implicated in the regulation of cellular growth, differentiation, and apoptosis, with dysregulation linked to cancer, cardiovascular disorders, and autoimmune conditions.

Detection is performed by a colorimetric method, with a measurement range of 15.6-1000pg/mL and a sensitivity of 8.08pg/mL. The assay is specific for natural and recombinant human SMAD4 and accepts serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Reported precision includes an intra-assay CV of 7.5% and an inter-assay CV of 11.7%, with recovery averaging 91% in serum (range 85-97%) and 93% in plasma (range 87-99%). The kit is for research use only and relates to UniProt entry Q13485.