Human Mothers against decapentaplegic homolog 3 (SMAD3) ELISA Kit

From Assay Genie, this sandwich ELISA kit is designed for the precise quantitative measurement of Mothers against decapentaplegic homolog 3 (SMAD3) in human samples, using a colorimetric detection method. Listed synonyms for the target include JV15-2, SMAD family member 3, MAD homolog 3 and MADH3.

SMAD3 is a receptor-regulated SMAD (R-SMAD) that functions as an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. Its dysregulation has been implicated in cancer, fibrosis and inflammation, supporting its value as a biomarker. The kit shows specificity for natural and recombinant human SMAD3 and is positioned within the cancer research area. The associated UniProt entry is P84022, and the protein is localised to the cytoplasm and nucleus.

The assay reports a detection range of 0.625-40ng/mL, a sensitivity of 0.159ng/mL, an intra CV of 3.5% and an inter CV of 6.2%. Recovery is reported as 91% (range 85-97%) for serum and 93% (range 87-99%) for plasma. Compatible sample types include serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. For research use only.