Human MOB2 (MOB kinase activator 2) ELISA KIT (HUFI05045)

This Assay Genie ELISA kit enables quantitative measurement of MOB kinase activator 2 (MOB2) in human biological specimens, supporting investigations of Hippo signaling and NDR kinase pathway regulation. MOB2 functions as an activating partner for NDR1/2 and LATS1/2 kinases, and altered expression has been examined in studies of cell cycle progression, centrosome duplication, and tumor cell motility. Researchers studying these mechanisms can profile MOB2 levels across cell lysates and tissue-derived preparations using this immunoassay.

Configured for in vitro research use only, the kit provides pre-coated microplate wells, matched detection antibodies, and a set of calibration standards suitable for absorbance-based quantification on a standard plate reader. Reaction develops via a chromogenic substrate producing a measurable color change, and absorbance values are recorded at the wavelength appropriate for the supplied chemistry. Reagents are supplied in sufficient quantity for the full plate format and arrive with a packaged insert detailing recommended diluents, incubation times, and curve-fitting guidance for interpolating unknown samples.

Typical applications include biomarker discovery in oncology research, pathway interrogation in cell signaling studies, and characterization of MOB family member expression in engineered cell lines or patient-derived primary material. The assay supports research into how MOB2 modulates downstream kinase activity and contributes to cellular proliferation and migration phenotypes. Reagents should be stored refrigerated between runs and equilibrated to ambient conditions before opening to preserve antibody binding activity across repeated use.