Human MMP14 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit offers high sensitivity and specificity for accurately quantifying MMP14 in human samples. Matrix metalloproteinase 14 (MMP14) is a key enzyme involved in extracellular matrix remodeling and plays a crucial role in cell migration, tissue repair, and tumor invasion. Abnormal MMP14 expression has been linked to conditions such as cancer, arthritis, and cardiovascular diseases, making it a valuable biomarker for studying disease mechanisms and exploring potential therapeutic targets.

The double-antibody sandwich assay uses colorimetric detection via TMB substrate, with absorbance read at 450 nm. It offers a sensitivity of 18.75 pg/mL and a working range of 31.25–2000 pg/mL. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with the kit intended for storage at 2–8°C for 12 months.

The procedure follows a standard sandwich ELISA workflow: reagent and plate preparation, primary incubation at 37°C for 90 minutes, biotinylated detection antibody binding, HRP-streptavidin binding, TMB color development in the dark for 10–20 minutes, and stop solution with absorbance measurement. Linearity has been demonstrated in serum, EDTA plasma, and heparin plasma across multiple dilutions, with recovery rates of 88–104% in serum, 85–101% in EDTA plasma, and 87–104% in heparin plasma.