Human MMP-9 (Matrix Metalloproteinase 9) CLIA Kit (HUES00828)

From Assay Genie, this chemiluminescence-based CLIA Kit provides a highly sensitive and specific assay for the precise quantification of human Matrix Metalloproteinase 9 (MMP-9) levels in serum, plasma, and cell culture supernatants. MMP-9 is a key enzyme involved in extracellular matrix remodeling, cell migration, and tissue repair, and it plays a role in inflammation, wound healing, cancer metastasis, and cardiovascular diseases, making it a valuable biomarker for research into disease progression and prognosis.

The assay follows a Sandwich-CLIA principle on a micro CLIA plate pre-coated with an antibody specific to Human MMP-9. Standards or samples are added to the wells, followed successively by a biotinylated detection antibody specific for MMP-9 and an Avidin-Horseradish Peroxidase (HRP) conjugate. After washing, a substrate solution is added; the resulting relative light unit (RLU) value is measured by a chemiluminescence immunoassay analyzer and is positively correlated with the concentration of Human MMP-9. The kit recognizes Human MMP-9 in samples, with no significant cross-reactivity or interference observed between MMP-9 and analogues.

Compatible sample types include serum, plasma, and other biological fluids, with a recommended sample volume of 100μL. The assay has a sensitivity of 37.50 pg/mL and a detection range of 62.50-4000 pg/mL, with a total assay time of 3.5h. Both intra-CV and inter-CV are reported as <15%. The kit applies to the in vitro quantitative determination of Human MMP-9 concentrations in serum, plasma, and other biological fluids.