Human MMP-25 (Matrix Metalloproteinase 25) ELISA Kit (HUES01408)

MMP-25, also designated MT6-MMP or leukolysin, is a membrane-type matrix metalloproteinase implicated in extracellular matrix turnover, leukocyte trafficking, and inflammatory cascades. The Assay Genie Sandwich ELISA quantifies human MMP-25 in serum, plasma, and other biological fluids using a colorimetric readout on a 96-well microplate pre-coated with capture antibody. Each kit contains recombinant MMP-25 standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, TMB substrate, stop solution, and adhesive plate sealer. Researchers apply the assay to investigate neutrophil activation, tumor invasion, tissue remodeling, and biomarker discovery in cardiovascular, inflammatory, and oncological research. Reconstituted standards and detection antibody should be aliquoted and stored frozen at -20°C, while the sealed microplate remains stable at 2-8°C and protected from light. The procedure typically completes within 3-4 hours and accepts sample volumes of 50-100 ul per well. Intended for research use only.