Human MMP-19 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit uses a double-antibody format and colorimetric detection to quantify MMP-19 (matrix metalloproteinase-19) in human samples. The kit accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Reported performance characteristics include a sensitivity of 0.469 ng/mL and an assay range of 0.781–50 ng/mL. Linearity across 1:2, 1:4, and 1:8 dilutions spans 85–105% for serum, 83–101% for EDTA plasma, and 83–100% for heparin plasma (n=5). Spike-and-recovery averages are 92% for serum (range 87–105%), 91% for EDTA plasma (range 86–98%), and 97% for heparin plasma (range 94–102%). The kit is stored at 2–8°C for 12 months and is positioned for cardiovascular research, reflecting MMP-19's known role in extracellular matrix remodeling and tissue repair and its reported dysregulation in cancer, arthritis, and fibrosis.

The protocol proceeds through plate and reagent equilibration, a 37°C primary incubation for 90 minutes, biotinylated detection antibody binding at 37°C for 60 minutes, HRP-streptavidin binding at 37°C for 30 minutes, TMB color development in the dark for 10–20 minutes, and a stop reaction with absorbance read at 450 nm.