Human Mitogen-activated protein kinase 14 (MAPK14) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the accurate measurement of Mitogen-activated protein kinase 14 (MAPK14) levels in human samples, using a colorimetric detection method. The kit is validated for serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. MAPK14 is described as a key signaling protein involved in cell growth, differentiation, and apoptosis, with dysregulation implicated in cancer, inflammatory conditions, and neurodegenerative disorders, making it a valuable biomarker for research. The target is also known by synonyms including p38 alpha, SAPK2a, CSBP, and MXI2.

Performance characteristics include a detection range of 0.156-10ng/mL, a sensitivity of 0.098ng/mL, an intra-assay CV of 6.3%, and an inter-assay CV of 10.8%. Linearity in serum (N=5) across dilutions 1:2, 1:4, 1:8, and 1:16 is 101-111%, 97-109%, 93-103%, and 94-106% respectively; EDTA plasma returns 109-117%, 100-109%, 106-116%, and 92-102%, while heparin plasma returns 102-111%, 85-97%, 105-117%, and 92-102%. Recovery averages 88% (range 82-94%) for serum and 90% (range 84-96%) for plasma. Specificity covers natural and recombinant human MAPK14. The kit is designated for research use only, with applications in the Neurosciences research area.