Human Mitoferrin (MFRN) ELISA Kit

Mitoferrin-1 (SLC25A28, also known as MFRN) is a mitochondrial inner-membrane iron transporter essential for mitochondrial iron import, heme biosynthesis, and iron-sulfur cluster biogenesis. The Human Mitoferrin ELISA Kit from Assay Genie provides quantitative measurement of this protein in human research samples, supporting studies of iron metabolism disorders, sideroblastic anemia, erythroid maturation, and mitochondrial bioenergetics.

This research-use-only kit is supplied in a 96-assay microplate format with colorimetric readout. Validated sample types include tissue homogenates and lysates commonly prepared for mitochondrial research. Each kit contains a pre-coated plate, reference standards, detection antibody, substrate, wash buffer, and stop solution required to complete the run.

The target protein is highly expressed in erythroid precursors and hematopoietic tissues, making the kit relevant for in vitro work on red blood cell development, in vivo models of iron overload, and translational research on disorders of mitochondrial iron handling. Researchers can pair the assay with western blot or PCR approaches to cross-validate expression changes across experimental conditions.

Storage and handling: keep reagents refrigerated upon receipt and avoid repeated freeze-thaw cycles to preserve antibody activity. Always consult the supplied protocol for recommended dilutions of tissue lysates and detailed procedural notes.