Human MIP-3 alpha PharmaGenie ELISA Kit (SBRS0886)

CCL20, also designated MIP-3 alpha, is a small chemokine of approximately 8 kDa that signals exclusively through the CCR6 receptor and is implicated in mucosal homing of lymphocytes and dendritic cells, inflammatory bowel disease, psoriasis, rheumatoid arthritis, and certain solid tumor microenvironments.

The Human MIP-3 alpha PharmaGenie ELISA Kit from Assay Genie is a quantitative in vitro immunoassay validated specifically for the measurement of endogenous human CCL20 concentrations across cell culture supernatants, cell lysates, blood-derived fractions, and tissue homogenates, supporting immunology, oncology, and inflammation research workflows.

The kit is supplied as a complete reagent set including a pre-coated microplate, lyophilized recombinant human CCL20 standards, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, and wash buffer sufficient for 96 individual determinations, with no significant cross-reactivity to unrelated murine chemokines. Reconstituted standards should be aliquoted and stored frozen at -20°C to limit freeze-thaw degradation, while the sealed plate and working reagents remain refrigerated between uses.

Researchers commonly apply this assay to conditioned media from stimulated peripheral blood mononuclear cells, inflamed mucosal biopsies, and tumor explant cultures to profile chemokine gradients and downstream CCR6-driven signaling responses. For research use only; not intended for diagnostic procedures.