Human MIP-1 alpha/CCL3 ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is designed for the precise detection of human MIP-1 alpha (CCL3) levels in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay uses a double-antibody sandwich format with colorimetric detection, providing high sensitivity and specificity for research applications.

Key performance specifications include a sensitivity of 14.063 pg/mL, a measuring range of 23.438–1500 pg/mL, human reactivity, and a recommended storage condition of 2–8 °C for 12 months. The analyte, MIP-1 alpha (also known as CCL3, Macrophage Inflammatory Protein 1 Alpha, LD78ALPHA, MIP1A, SCYA3, or G0S19-1; Uniprot P10147), is a chemokine central to immune response, inflammation, and cell recruitment, with established roles in autoimmune, infectious, and inflammatory disease research.

The workflow follows a six-step protocol involving reagent and plate preparation, primary incubation, biotin-labeled detection antibody binding, HRP-Streptavidin (SABC) binding, TMB colour development, and absorbance reading at 450 nm. Validated linearity and recovery values are reported for serum, EDTA plasma, and heparin plasma matrices, supporting reliable quantitation across a range of sample conditions.