Human mIgM ELISA Kit

From Assay Genie, this Human mIgM (Membrane Immunoglobulin M) ELISA Kit is a sandwich ELISA designed for the precise measurement of mIgM levels in human biological samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. MIGM, also known as Membrane-Bound IgM, is described as a component of the immune system involved in the recognition and elimination of pathogens, with elevated levels linked to autoimmune disorders, infectious diseases, and inflammatory conditions, supporting its use as a biomarker in research.

Performance specifications include a sensitivity of 1.875 ng/mL and a standard range of 3.125-200 ng/mL, using a colorimetric double antibody sandwich detection method. Storage is at 2-8°C for 12 months. Validated linearity in serum (n=5) spans 88-92% at 1:2, 87-100% at 1:4, and 91-101% at 1:8 dilution, with EDTA plasma and heparin plasma linearity also reported. Recovery for serum is 95-105% (average 99%), EDTA plasma 86-100% (average 95%), and heparin plasma 86-105% (average 94%).

The assay procedure involves equilibrating reagents and TMB substrate, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding for 60 minutes, HRP-Streptavidin binding for 30 minutes, TMB color development in the dark for 10-20 minutes, and absorbance measurement at 450 nm after stop solution addition.