Human MICB (MHC class I polypeptide-related sequence B) ELISA Kit (HUFI07618)

Sandwich ELISA Kit from Assay Genie that quantifies human MICB (MHC class I polypeptide-related sequence B), a stress-induced NKG2D ligand shed from epithelial and tumor cells during viral infection, DNA damage, and malignant transformation. Specificity testing confirmed no detectable cross-reactivity with other relevant proteins, and the matched antibody pair recognizes both natural and recombinant human MICB. The kit supplies a pre-coated microplate, lyophilized recombinant standards, biotinylated detection antibody, HRP-streptavidin conjugate, TMB substrate, wash buffer, and dilution buffers sufficient for 96 determinations. Components are stored refrigerated at 2-8°C protected from light, with a 12-month shelf life from manufacture. The protocol uses a 100 µL sample volume per well and produces a colorimetric readout on a standard microplate reader. For research use only — not intended for clinical or diagnostic procedures. Researchers commonly combine this measurement with flow cytometry analysis of NKG2D receptor expression on NK and CD8+ T cell subsets, or with immunohistochemistry of tumor biopsies to relate circulating soluble MICB to residual membrane-bound ligand staining. Compatible matrices include serum samples, plasma, tissue homogenates, and cell culture supernatant.