Human MICA PharmaGenie ELISA Kit (SBRS0879)

The Human MICA ELISA Kit from Assay Genie provides quantitative measurement of MHC class I chain-related protein A (MICA), a stress-induced NKG2D ligand under active investigation in tumor immunology, transplant rejection, and autoimmune disease research. MICA expression is upregulated on epithelial, fibroblast, and tumor cell surfaces following cellular stress, viral infection, or DNA damage, while proteolytic shedding generates soluble MICA (sMICA) that suppresses NKG2D-mediated NK cell activation—a mechanism linked to immune evasion across solid tumors and hematologic malignancies.

Researchers commonly deploy this microplate-based assay to quantify MICA in cell culture supernatant, plasma, or serum samples, supporting studies of NK and CD8+ T cell biology, NKG2D signaling pathways, graft-versus-host disease, and serum biomarker profiling in hepatocellular carcinoma, breast, lung, and colorectal cancers. The kit includes pre-coated capture antibody wells, lyophilized recombinant standards, biotinylated detection reagent, streptavidin-HRP conjugate, and assay buffers; reagents should be stored at -20 °C with aliquoted standards to limit freeze-thaw loss. The standard protocol uses 100 µL of sample per well, and the assay is validated specifically for human MICA with negligible cross-reactivity to murine MICA or the related NKG2D ligand MICB. Intended for in vitro research use only.