Human MGAT4A ELISA Kit (HUFI07624)

MGAT4A is a Golgi-resident glycosyltransferase that catalyzes the transfer of N-acetylglucosamine to the alpha-1,3-mannose arm of N-linked glycans, determining whether biantennary glycans are extended into tri- or tetra-antennary structures. The Assay Genie Human MGAT4A ELISA Kit enables quantitative measurement of this enzyme in human research specimens.

This protein has drawn attention in glycobiology because its branching activity modulates receptor signaling at the cell surface. Published studies have linked MGAT4A expression to insulin receptor sensitivity and to altered adhesion-molecule behavior in epithelial tumor models, making reliable quantification a useful biomarker for research into glucose homeostasis, oncogenic transformation, and aberrant glycosylation patterns.

The kit is configured in a 96 Assays microplate format, supporting parallel processing of standards, controls, and biological samples in a single run. It is supplied as an immunoassay package with all reagents needed to perform the assay and is intended for research use only, validated specifically for human reactivity.

Common applications include comparing MGAT4A abundance between disease and control cohorts, tracking protein-level changes in cell culture models following gene knockdown or overexpression, and screening compound libraries for modulators of N-glycan branching. Researchers interested in MGAT4A enzymatic activity or transcript levels should note that this ELISA detects the protein product, providing a complementary readout to enzymatic and qPCR-based analyses.