Human Methylated-DNA--protein-cysteine methyltransferase (MGMT) ELISA Kit

Assay Genie provides a Sandwich ELISA for the quantitative detection of human Methylated-DNA--protein-cysteine methyltransferase (MGMT), a nuclear DNA repair enzyme that stoichiometrically transfers alkyl groups from the O6 position of guanine to a cysteine residue within itself in a suicide-like reaction. The colorimetric assay is validated for use with human serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, and is specific for natural and recombinant human MGMT. The product is intended for research use only.

Performance characteristics include a detection range of 0.156-10ng/mL and a sensitivity of 0.049ng/mL, with an intra-assay CV of 6.5% and an inter-assay CV of 10.2%. Linearity has been assessed across dilutions of 1:2, 1:4, 1:8, and 1:16 in serum, EDTA plasma, and heparin plasma. Recovery averages 102% in serum (range 96-108%) and 104% in plasma (range 98-110%).

MGMT functions in cellular defense against the biological effects of O6-methylguanine in DNA, and alterations in its expression have been linked to cancer and neurological disorders, making it a relevant biomarker for oncology research. The protein is localized to the nucleus, carries UniProt accession P16455, and has a molecular weight of 21,646 Da. Synonyms listed for the target include 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase, and MGMT.