Human Metallothionein 1M ELISA Kit

From Assay Genie, this sandwich ELISA kit (double-antibody format) is engineered for the quantitative measurement of Metallothionein 1M (MT1M) in human samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The target analyte is captured on a pre-coated 96-well microplate via an immobilised antibody, then detected using a biotinylated antibody followed by HRP-conjugated Streptavidin. Background material describes Metallothionein 1M as a vital protein with roles in metal ion homeostasis, heavy metal detoxification, and protection against oxidative stress, with research relevance as a biomarker in cancer, neurodegenerative diseases, and metal toxicity.

Reported performance values include a detection range of 3.125-200 ng/mL and a sensitivity of 1.875 ng/mL. The colourimetric signal is generated by TMB substrate (blue product that turns yellow after stop solution) and absorbance is read at 450 nm, with values directly proportional to analyte concentration through a standard curve. Kit reagents comprise a lyophilised standard, biotinylated detection antibody (100X), HRP-Streptavidin conjugate SABC (100X), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, 25X wash buffer, stop solution, plate sealer, technical manual, and Bradford Reagent for total protein normalisation. The kit is shipped on ice packs with reagents stored at 2-8°C for 12 months.

Validation data report mean serum recovery of 93% (range 85-101%), EDTA plasma mean 95% (range 88-100%), and heparin plasma mean 95% (range 88-105%). Dilutional linearity (1:2 / 1:4 / 1:8) was 88-104% / 87-105% / 91-102% for serum, 85-100% / 84-95% / 83-101% for EDTA plasma, and 87-100% / 82-94% / 81-86% for heparin plasma. The kit is for research use only.