Human MEPE/Matrix extracellular phosphoglycoprotein ELISA Kit

A Sandwich ELISA Kit from Assay Genie designed for the quantitative detection of Human MEPE (Matrix Extracellular Phosphoglycoprotein). The assay uses a pre-coated microplate and a biotin-conjugated antibody, followed by Streptavidin-HRP and TMB substrate, with absorbance read at 450 nm. Reported synonyms for the target include OF45, Asarm Motif-Containing, and Osteoblast/Osteocyte Factor 45.

Validated sample types include serum, plasma, tissue homogenates, cell lysates, and other biological fluids. Performance characteristics stated by the manufacturer include a sensitivity of 0.139 ng/mL, an assay range of 0.32-20 ng/mL, a standard concentration of 20 ng/mL, and a total assay length of 3.5h. Linearity in serum is reported as 85-102% (1:2), 82-96% (1:4), 87-98% (1:8), and 78-92% (1:16); in EDTA plasma 98-105%, 86-92%, 91-103%, and 89-97%; and in heparin plasma 95-106%, 87-96%, 82-90%, and 92-101%. Spike-and-recovery averages are 92% in serum, 88% in EDTA plasma, and 87% in heparin plasma. Precision is reported as intra-assay CV%<8% and inter-assay CV%<10%.