Human Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) ELISA Kit

Quantitative measurement of human melanoma-associated chondroitin sulfate proteoglycan (MCSP), also called CSPG4, in research samples is supported by this immunoassay kit from Assay Genie. MCSP is a transmembrane chondroitin sulfate proteoglycan first identified on melanoma cells and now investigated as a surface marker of mesenchymal stem cells, pericytes, and certain tumor-initiating populations. Researchers working on melanoma progression, tumor angiogenesis, stromal interactions, and progenitor-cell characterization use this kit to obtain reproducible readouts across biological replicates.

Validated for use with tissue homogenates and cell lysates, the kit accommodates studies in which cultured cells or freshly prepared tissue specimens are characterized. Each pack provides materials for 96 assays and is intended for in vitro research applications. The assay relies on pre-coated capture antibodies paired with a biotinylated detection antibody and HRP-streptavidin, producing a measurable colorimetric signal read on a standard microplate reader after substrate addition and stop solution.

Typical laboratory workflows include profiling MCSP expression across panels of melanoma cell lines, comparing levels between primary tumor tissue and matched normal controls, characterizing pericyte or mesenchymal stem cell cultures, and monitoring MCSP abundance after gene knockdown, pharmacological treatment, or differentiation protocols. Unopened components should be refrigerated until first use, then handled according to the enclosed protocol to preserve antibody activity and ensure lot-to-lot consistency.