Human Melanoma-Associated Antigen 4 (MAGEA4) ELISA Kit

The MAGEA4 protein belongs to the cancer-testis antigen family, normally restricted to testicular germ cells and placenta in healthy adults but aberrantly expressed across melanoma, non-small cell lung, and head-and-neck tumors, where it serves as both a diagnostic biomarker and an immunotherapeutic target. Researchers use this Assay Genie Sandwich ELISA to quantify MAGEA4 levels in serum, plasma, tissue homogenates, and other biological fluids, supporting studies of tumor antigen re-expression, immune monitoring, and biomarker discovery. The kit is supplied in a 96-well microplate format with colorimetric readout, and unopened components should be refrigerated at 2-8 °C to preserve antibody activity. The capture antibody is raised against the human MAGEA4 sequence, with no significant cross-reactivity reported against related MAGE family members such as MAGEA1, MAGEA2, MAGEA3, or MAGEB2. Each kit contains a pre-coated plate, calibrated standards, biotinylated detection antibody, streptavidin-HRP conjugate, and chromogenic substrate sufficient for 96 assays, making it well suited for longitudinal screening of patient cohorts and tumor xenograft studies.