Human Melanoma antigen preferentially expressed in tumors (PRAME) ELISA Kit

Assay Genie's colorimetric Sandwich ELISA Kit is designed for the precise measurement of PRAME (Melanoma antigen preferentially expressed in tumors) in human samples, including serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. PRAME is a protein preferentially expressed in melanoma tumors and is recognised by cytolytic T lymphocytes, making it a valuable biomarker for melanoma detection and monitoring. Its expression has also been linked to various other cancer types, supporting its use as a versatile tool in oncology research.

The assay delivers a detection range of 0.156–10 ng/mL and a sensitivity of 0.056 ng/mL, with an intra-assay CV of 4.9% and inter-assay CV of 8.8%. Specificity is directed against natural and recombinant human PRAME. Linearity has been validated in serum, EDTA plasma and heparin plasma across serial dilutions from 1:2 to 1:16, and recovery averages 92% for serum and 94% for plasma. The target functions as a transcriptional repressor of retinoic acid signaling through receptors RARA, RARB and RARG, and is localised to the nucleus and cell membrane. For research use only.