Human Melan-A/MART-1 ELISA Kit

From Assay Genie, this Sandwich ELISA, Double Antibody kit is designed for the precise measurement of Melan-A/MART-1 levels in human samples, supporting research into melanoma progression, prognosis, and treatment response. The kit accepts a broad range of sample types, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Performance characteristics reported by the manufacturer include a sensitivity of 46.875 pg/mL and an assay range of 78.125-5000 pg/mL. Linearity across 1:2, 1:4, and 1:8 dilutions is reported as 85-100%, 89-104%, and 86-105% for serum (n = 5); 88-101%, 86-100%, and 82-100% for EDTA plasma (n = 5); and 89-100%, 83-100%, and 84-97% for heparin plasma (n = 5). Recovery is reported as 85-105% (average 92%) for serum, 89-102% (average 95%) for EDTA plasma, and 85-102% (average 97%) for heparin plasma. Detection is colorimetric, with absorbance read at 450 nm after TMB substrate development.

Kit components include a dismountable ELISA microplate, lyophilized standard, biotin-labeled antibody (100X), HRP-Streptavidin conjugate (SABC, 100X), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, wash buffer (25X), plate sealer, and a technical manual. Storage is 2-8°C for 12 months. The kit is intended for research use in cell biology applications.