Human MEK5 ELISA Kit

Manufactured by Assay Genie, this sandwich ELISA kit enables quantitative measurement of human MEK5 (Mitogen-Activated Protein Kinase Kinase 5), a key enzyme in the MAPK signaling pathway that plays a critical role in cell growth, differentiation, and survival. The assay uses a double-antibody sandwich format with colorimetric detection, delivering a sensitivity of 0.188 ng/mL and an analytical range of 0.313–20 ng/mL.

Validated for human samples, the kit is suitable for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types. It is intended for cell biology research, where dysregulation of MEK5 has been implicated in cancer and inflammatory disorders, highlighting its value as a potential biomarker and therapeutic target for understanding disease mechanisms and progression.

Reagents should be stored at 2–8 °C, where the kit remains stable for 12 months. Manufacturer-reported validation demonstrates robust linearity, typically 80–105 % across tested dilutions, and recovery values averaging 95–97 % in serum, EDTA plasma, and heparin plasma, supporting reliable performance across multiple sample matrices.