Human MED6 ELISA Kit (HUFI07636)

The MED6 target protein (NCBI Gene ID 10001, UniProt O75586) is a 246-amino-acid, 28 kDa nuclear factor encoded at chromosomal locus 14q24.2 and historically tracked under the aliases ARC33 and NY-REN-28. As a structural member of the Mediator head module, MED6 contacts MED1, MED4, MED7, MED17, MED21 and MED31 directly and forms the scaffold on which the pre-initiation complex assembles, relaying signals from gene-specific activators to RNA polymerase II. Published interaction mapping has placed MED6 alongside hormone receptors (VDR, RARA), the BCL6 transcriptional repressor, the SMARCA4 chromatin remodeler, and the CDK8/CDK19 kinase module, and it was originally identified as a renal carcinoma antigen (NY-REN-28) in serological screens.

Quantitative measurement of endogenous MED6 with this Assay Genie kit supports research into Mediator-dependent transcriptional dysregulation, signal-dependent gene activation, and the role of MED6 stoichiometry in cancer biology and developmental disorders. The kit provides a pre-coated strip-well microplate, lyophilized reference standards, and matched detection reagents, and the standard curve is built from a recombinant human MED6 calibrator. Reconstituted components should be aliquoted and stored frozen to limit freeze-thaw cycles, while the working microplate is held at 4°C between incubations to preserve antibody binding activity. The MED6 polyclonal antibody offered by Assay Genie for the same immunogen is validated for western blot and ELISA on MCF7, Jurkat and HeLa lysates, allowing orthogonal confirmation of any unusual signal.