Human MED17 ELISA Kit (HUFI07652)

The Human MED17 ELISA Kit from Assay Genie provides a quantitative immunoassay for measuring Mediator Complex Subunit 17, a core component of the Mediator complex that regulates RNA polymerase II-dependent transcription of protein-coding genes. MED17 (also known as CRSP6) is essential for embryonic development and has been linked through clinical studies to microcephaly, intellectual disability, and post-translational histone modification defects when mutated.

This assay is configured and validated for detection of human MED17, with no significant cross-reactivity reported against mouse or rat homologs under the standard protocol. Investigators studying transcriptional regulation, stem cell differentiation, or Mediator-related disorders can use this plate-based assay to quantify MED17 protein in research specimens. The kit supplies pre-coated wells, standard curve calibrators, biotinylated detection reagent, and chromogenic substrate sufficient for 96 determinations. For optimal reagent activity, store kit components at refrigerated temperature and avoid repeated freeze-thaw cycles of the standard and detection antibody. A standard microplate reader capable of absorbance measurement is required, and the complete protocol can be finished within a typical workday.