Human MED15 ELISA Kit (HUFI07651)

Built by Assay Genie, this ELISA kit quantitatively measures Mediator Complex Subunit 15 (MED15, also known as ARC105 or TIG-1), a core component of the multiprotein Mediator complex that conveys regulatory signals from DNA-bound transcription factors to RNA polymerase II during transcription initiation. MED15 has been linked to head and neck squamous cell carcinoma, bladder cancer, and altered SREBP-driven lipid metabolism, making it a target of interest in cancer biology, endocrinology, and gene-expression research.

The kit supplies a pre-coated microplate, lyophilized recombinant human MED15 standards, a biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, and TMB substrate. Stock reagents should be kept refrigerated until first use; reconstituted standards must be aliquoted and frozen at -20 degrees C, with repeated freeze-thaw cycles avoided to preserve antibody binding activity. Bring all components to room temperature before initiating the protocol.

Typical research applications include biomarker discovery, nuclear receptor signaling investigations, Mediator complex functional studies, and comparative analysis of clinical patient cohorts. The assay provides reproducible standard curves and is intended for research use only in laboratory settings, supporting translational programs that probe transcriptional dysregulation in vitro and in disease-relevant tissues.