Human MED12L ELISA Kit (HUFI07650)

This Assay Genie kit delivers quantitative immunoassay measurement of human MED12L (Mediator Complex Subunit 12-Like), a paralog of MED12 that participates in the multi-subunit Mediator complex governing RNA polymerase II transcription. Researchers studying transcriptional regulation, cancer biology, or neurodevelopment can apply it to probe MED12L abundance in cell lysates and tissue homogenates prepared from human samples. The product ships with reagents for 96 determinations, including a pre-coated plate, detection antibody, standards, and the buffers required to run the protocol on a standard microplate reader.

Because MED12L has been linked to specific leukemia translocations and to certain developmental disorders, the kit is most useful in experimental workflows that compare MED12L abundance between sample cohorts—for example, tumor versus matched normal tissue, or patient-derived lines with and without MED12L perturbation. The readout is a colorimetric signal proportional to MED12L concentration in each well. Working standards should be diluted on the day of use, and any unused standard should be aliquoted into single use vials to limit freeze-thaw cycles on the stock. Reconstituted and diluted reagents should be handled according to the insert; keep sealed components stored as printed on the label to preserve activity through the stated shelf life.

This product is supplied for research use only and is not validated for diagnostic or clinical decision-making.