Human ME20M (Melanoma Associated ME20) ELISA Kit (HUES03290)

ME20M corresponds to premelanosome protein (PMEL), also annotated as GP100, Pmel17, SIL, SILV, and Silver homolog — a 95 kDa melanocyte-specific glycoprotein involved in melanosome biogenesis. Mutations in the corresponding gene are linked to pigmentary phenotypes, while its overexpression in melanoma makes it a target of interest for tumor immunology studies. The Assay Genie sandwich ELISA relies on a biotin-conjugated detection antibody and Avidin-HRP, with TMB substrate development and a sulphuric acid stop solution; absorbance is read at 450 nm ± 10 nm. Standard curves typically span 0.156–10 ng/mL, with a minimum detectable dose around 0.056 ng/mL. The reported intra-assay CV is below 10% and inter-assay CV below 12%, supporting reproducible quantitation across plates. Compatible sample matrices include serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants. Reagents should be kept refrigerated at 2–8 °C, with the standard and antibody components stable for the shelf life indicated on the vial. Researchers studying pigment cell differentiation, melanoma biomarker validation, or immunotherapy response monitoring will find this 96-well format straightforward to integrate into existing plate-based workflows.